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  • 產(chǎn)品名稱： Biotin-14-dATP
  • 產(chǎn)品貨號： CSC431
  • 貨期： 現(xiàn)貨
  • 價格與訂購： 3800
  • 數(shù)量：
    庫存： 5
  • 規(guī)格： 50nmol
  • 產(chǎn)品信息
  • 如何訂購
    Introduction
    Biotin-14-dATP is a dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker. The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids in in situ and filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP. Biotin-14-dATP can be added to the 3′ end of DNA using terminal deoxynucleotidyl transferase. The amount supplied is sufficient for labeling up to 50 μg of DNA by nick translation.
    Applications
    Incorporation into DNA/cDNA by
     
    – Nick Translation with DNAse I/ DNA Polymerase I 
     
    – Primer Extension with Klenow fragment
    Specifications
    Concentration: 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA.
    Storage: -20°C
    Product size: 50 nmol
    References
    Novel biotinylated nucleotide-analogs for labeling and colorimetric detection of DNA.
    Gebeyehu et al.
    Nucleic Acids Res. (1987) 15 (21):4513
    Single-cell Hi-C for genome-wide detection of chromatin interactions that occur simultaneously in a single cell.
    Nagano et al.
    Nature Protocols (2015) 10 (12):1987.
    HiChIP: Efficient and sensitive analysis of protein-directed genome architecture.
    Mumbach et al.
    Nature Protocols (2016) 13 (11):919.
    Note
    For research use only. Not suitable for clinical or therapeutic use.